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Health Lab Test Code Build Information
Test Name:	ANA SCREEN/REFLEX TITER, REFLEX DNA/ENA-HealthLab Only
Test Code:	2320099
Alias:	ANA LAB147
CPT Code(s):	86038
Test Includes:	If ANA screen is positive, ANA titer/pattern and specific autoantibodies are performed. Specific autoantibodies include dsDNA, SSA, SSB, SM (Smith), SM/RNP, Scleroderma 70 (SLC-70), and Jo-1 will be performed. If reflex testing is performed, additional charges apply (reflex 86235x6, 86225, 86039).
Preferred Specimen:	1.0 mL serum
Container:	SST (gold)
Alternate Container:	Plain Red (no additive/gel)
Minimum Volume:	0.5 mL serum
Collection Instructions:	Allow specimen to completely clot (this can take up to 60 minutes). Centrifuge for 15 minutes as soon as the specimen is clotted. If the specimen is collected in a Plain Red (no additive/gel) tube, transfer the separated serum to a properly labeled plastic transfer tube. Specify specimen type (serum) on transfer tube.
Transport Temperature:	Refrigerated
Stability:	Refrigerated: 7 days Frozen (-20 degrees C or colder) for longer storage
Rejection Criteria:	Gross hemolysis Gross lipemia High degree of microbial growth
Additional Information:	For reflex test information, see the individual tests.
Schedule:	Testing performed: Monday-Friday; Results in 1-3 days
Method:	Immunofluorescence assay (IFA)
Clinical Significance:	The antinuclear antibody (ANA) test is an indirect immunofluorescent test to detect antinuclear antibody activity in sera of patients with autoimmune rheumatic disease and connective tissue disease.
Use:	updated 10/13/25
Reference Ranges:	Negative

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	The CPT codes included in this publication are in accordance with Current Procedural Terminology, a publication of the American Medical Association. CPT codes are provided here for the convenience of our clients; however, correct coding often varies from one carrier to another, and HealthLab may bill specific carriers using codes other than those shown. Clients who bill for services should verify the code(s) with the applicable payor to confirm that their use is appropriate in each case.
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