Test Name: *RETIRED* T LYMPHOCYTE HELPER/SUPPRESSOR PANEL
Test Code: 2199306
Alias: Helper:Suppressor Ratio
LAB9234
T4:T8 Analysis
CPT Code(s): 86359   86360  
Test Includes:

Absolute CD4 helper/inducer T cells (CD3+CD4+); absolute CD8 suppressor/cytotoxic T cells (CD3+CD8+); absolute lymphocyte count; absolute T cells (CD3); CD4:CD8 ratio; CBC with differential and platelet count

Preferred Specimen:

Whole blood

Container:

EDTA (lavender) AND ACD-A or ACD-B (yellow)

Fill tubes to capacity.

Collection Instructions:

Invert tube 8 to 10 times immediately after collection.

To preserve cellular viability, collect specimen so it will arrive in the laboratory within 48 hours of collection. Indicate date and time of venipuncture on the tube(s) and on the test request form.

Transport Temperature: Maintain specimen at room temperature. Do not freeze or refrigerate.
Stability:

Room temperature: 2 days
Refrigerated: Unacceptable  
Frozen: Unacceptable

Rejection Criteria:

Hemolysis; specimen refrigerated or frozen; clotted specimen; contaminated specimen

Limitations: Reference intervals for adults have been established by the laboratory.
Method: Flow Cytometry
Performing Lab: LabCorp; #096925
Clinical Significance: HIV-1 infection results in a decrease of CD4 T cells, an increase in CD8 T cells, a decrease in the CD4:CD8 ratio, and a progressive destruction of immune function. Enumeration of CD4 and CD8 T cells in HIV-1 seropositive patients may be used for prognostic purposes, to monitor disease progression and retroviral therapy.
Use: Updated 02/27/2021
Reference Ranges: See Laboratory Report

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