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Test Name:

VITAMIN D, 25-OH (TOTAL D2/D3)

Test Code:

2128210

Alias:

LAB3055
Vitamin D 25-OH, 25-Hydroxycalciferol, 25-OH-D, Cholecalciferol Metabolite, Vitamin D, Vitamin D3 Metabolite

CPT Code(s):

82306

Preferred Specimen:

1.0 mL serum

Container:

SST (gold)

Alternate Container:

Lithium Heparin (Lt green)

Minimum Volume:

0.3 mL serum
This volume does not allow for repeat testing.

Collection Instructions:

Allow specimen to completely clot (this can take up to 60 minutes). Centrifuge for 15 minutes. If the specimen is collected in a tube without a gel barrier, centrifuge and transfer the plasma into a properly labeled transfer tube within 2 hours of collection.

Transport Temperature:

Refrigerated

Stability:

Room Temperature: 72 hours

Refrigerated: 7 days

Frozen: 1 year

Rejection Criteria:

Gross hemolysis

Limitations:

Additional Information:

Falsely elevated results may occur in patients being treated with Paricalcitol (Zemplar). Vitamin D levels should not be tested in patients who have received Paricalcitol within 24 hours of obtaining the sample

Schedule:

Set up Daily; Results in 24-48 hours

Method:

Paramagnetic-particle, chemiluminescent immunoassay

Clinical Significance:

The role of vitamin D in bone and mineral metabolism was recognized from its first identification as a factor that could cure rickets. However, vitamin D is now recognized as a prohormone which has multiple roles in maintaining optimal health.

Vitamin D3 (cholecalciferol) and vitamin D2 (ergocalciferol) are the most abundant forms of vitamin D in the body. Vitamin D3 is synthesized in the skin in response to sunlight. The best nutrition sources of D3 are oily fish. Vitamin D2 nutrition sources are from some vegetables, yeast, and fungi. Vitamin D (D3, D2, and metabolites) is converted to 25-hydroxyvitamin D in the liver. The measurement of 25-OH vitamin D is the best indicator of vitamin D nutritional status.

Use:

Updated 6/27/2022

Reference Ranges:

Deficiency:              <20 ng/ml
Insufficiency:            20 – 29 ng/ml
Optimum Level:      30 – 100 ng/ml
Possible Toxicity:     >150 ng/ml

Reference Range Table

Result	Units	Gender	Age	Range
Vitamin D, 25-OH	ng/mL	Both	0 Year - 999 Year	30.0-80.0

The CPT codes included in this publication are in accordance with Current Procedural Terminology, a publication of the American Medical Association. CPT codes are provided here for the convenience of our clients; however, correct coding often varies from one carrier to another, and HealthLab may bill specific carriers using codes other than those shown. Clients who bill for services should verify the code(s) with the applicable payor to confirm that their use is appropriate in each case.

Reference Range Table

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