Test Name: LEUKOTRIENE E4, 24-HOUR URINE
Test Code: 2194141
Alias: LAB15309
CPT Code(s): 82542  
Test Includes: Quantitation of Leukotriene E4 and Creatinine in 24 hr urine collection
Preferred Specimen:

5 mL aliquot of a well-mixed 24 hr urine collection (no preservative)

Container:

BD no additive tube

Alternate Container:

Sterile urine cup

Minimum Volume:

2 mL

Collection Instructions:

Patient preparation: Patients taking the 5-lipoxygenase inhibitor zileuton (Zyflo) may have decreased concentrations of leukotriene E4 (LTE4) if the dosage has not been discontinued for 48 hours.

Start collection within a few hours of symptom onset and collect urine for 24 hours into a preservative free container. Refrigeration of specimen during collection is preferred. Record total volume. Aliquot 5 mL of a well-mixed, 24-hour urine collection into a BD no additive tube. Send frozen.  

Transport Temperature:

Frozen

Stability:

Room temperature: 24 hours

Refrigerated: 7 days

Frozen: 28 days

Limitations:

This test was developed, and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the US Food and Drug Administration.

Schedule:

Set up: Monday, Thursday; Report Available: 3-10 days

Method:

Leukotriene E4: Liquid Chromatography Tandem Mass Spectrometry (LC-MS/MS)

Creatinine: Enzymatic Colorimetric Assay

Performing Lab:

Mayo Clinic Lab; Test ID: TLTE4

Clinical Significance:

Elevated urinary leukotriene E4 (LTE4) concentrations above 104 pg/mg creatinine may be suggestive of mast cell activation syndrome if compatible features of disease are present.

Use:

Updated: 7/25/2023

Reference Ranges:

LEUKOTRIENE E4:

< or =104 pg/mg creatinine

 

CREATININE:

Normal values mg per 24 hours:

Males: 930-2955 mg/24 hours

Females: 603-1783 mg/24 hours

Reference values have not been established for patients who are younger than 18 years of age.

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The CPT codes included in this publication are in accordance with Current Procedural Terminology, a publication of the American Medical Association. CPT codes are provided here for the convenience of our clients; however, correct coding often varies from one carrier to another, and HealthLab may bill specific carriers using codes other than those shown. Clients who bill for services should verify the code(s) with the applicable payor to confirm that their use is appropriate in each case.

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