Test Name: FACTOR II GENE MUTATION
Test Code: 2322840
Alias: LAB834
CPT Code(s): 81240   G0452  
Preferred Specimen: 1.0 mL whole blood
Container: K2 or K3 EDTA anticoagulant (Lavender top)
Minimum Volume:

50uL

*Note: this volume does not allow for repeat testing.
Collection Instructions:

Venipuncture: Allow EDTA tube to fill completely. Gently invert tube 8-10 times IMMEDIATELY upon collection to prevent clotting. Only one lavender tube is needed.

Capillary: Agitate microtainer after EACH drop of blood until the upper line (500uL) is reached. Do NOT overfill. Add cap and forcibly invert tube thoroughly 10 times to prevent clotting. Attach plastic extender to microtainer and label.

Transport Temperature:

Refrigerated
Stability:

Room temperature: 24 hours (15-30ºC)

Refrigerated for up to 15 days (2-8ºC)

Frozen for up to 3 months (-20ºC or -80ºC)
Rejection Criteria: Frozen plasma, spun plasma (removed from cells)
Limitations: Patients on heparin therapy and blood transfusion patients may have blood specimens that potentially interfere with the PCR results and lead to invalid or erroneous results.
Additional Information:

All molecular genetic testing is required to have a Pathology review. Additional charges apply in addition to molecular genetic testing.

Schedule: Daily; Turnaround time: <= 24 hours
Method: real-time Polymerase Chain Reaction (PCR)
Performing Lab: HealthLab
Clinical Significance:

The association of Factor II (G20210A) and Factor V Leiden (G1691A) mutations with an increased risk for venous thrombosis has been well documented. The Factor II or Prothrombin (G20210A) mutation refers to the G to A transition at nucleotide 20210 in the 3' untranslated region of the gene and is associated with increased plasma levels of prothrombin. Factor V Leiden (G1691A) refers to the G to A transition at nucleotide position 1691 of the Factor V gene, resulting in the substitution of the amino acid arginine by glutamine in the Factor V protein, causing resistance to cleavage by Activated Protein C (APC). Factor II (G20210A) and Factor V Leiden (G1691A) mutations are present in 2% and 5% of the general population, respectively.
Department: Immunology
Reference Ranges: Normal

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The CPT codes included in this publication are in accordance with Current Procedural Terminology, a publication of the American Medical Association. CPT codes are provided here for the convenience of our clients; however, correct coding often varies from one carrier to another, and HealthLab may bill specific carriers using codes other than those shown. Clients who bill for services should verify the code(s) with the applicable payor to confirm that their use is appropriate in each case.
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