Test Name: PROTEIN ELECTROPHORESIS, SERUM
Test Code: 2120152
Alias: LAB119
SPE
CPT Code(s): 84165   84155  
Test Includes: PROTEIN ELECTROPHORESIS, SERUM 
PROTEIN, TOTAL SERUM 
Preferred Specimen:

2.0 mL serum

Container:

SST (gold)

Alternate Container: Plain Red (no additive)
Minimum Volume:

1.0 mL serum.

This volume does not allow for repeat testing.

Collection Instructions:

A fasting specimen is preferred. Allow specimen to completely clot (this can take up to 60 minutes). Centrifuge for 15 minutes. The serum should be physically separated from contact with cells within two hours from the time of collection. If the specimen is collected in a plain red (no additive) tube, centrifuge and transfer serum into a properly labeled transfer tube within two hours of collection.

Transport Temperature: Refrigerated
Stability:

Fresh samples recommended

Refrigerated: 7 days

Rejection Criteria:

Hemolysis

Plasma

Additional Information:

If a Serum Protein Electrophoresis (SPE) is ordered in conjunction with a Comprehensive Metabolic Panel (CMP), only the SPE component will be billed (Total Protein component is already billed as part of the CMP).

NOTE:  Immunofixation testing added on at the discretion of the Pathologist upon review of the serum electrophoresis results. Additional CPT charges will apply.

Schedule:

Set up Mon-Fri (excluding holidays); Results in 72 hours

Method:

Capillary Zone Electrophoresis

Rate Biuret (Total Protein)

Clinical Significance: The electrophoretic separation of serum proteins is a screening procedure and aids in the diagnosis of pathophysiologic states associated with changes in protein quantity and molecular nature.
Reference Ranges: Updated on 02/17/22

Reference Range Table

Result Units Gender Age Range
Alpha-1 gm/dL Both 0 Day - 999 Year 0.17-0.41
Alpha-2 gm/dL Both 0 Day - 999 Year 0.43-0.98
Beta-1 gm/dL Both 0 Day - 999 Year 0.28-0.60
Beta-2 gm/dL Both 0 Day - 999 Year 0.19-0.54
Gamma gm/dL Both 0 Day - 999 Year 0.67-1.56

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The CPT codes included in this publication are in accordance with Current Procedural Terminology, a publication of the American Medical Association. CPT codes are provided here for the convenience of our clients; however, correct coding often varies from one carrier to another, and HealthLab may bill specific carriers using codes other than those shown. Clients who bill for services should verify the code(s) with the applicable payor to confirm that their use is appropriate in each case.

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